1515 Geniouskin: The first pigmented artificial skin for clinical use

Skin replacement is of major concern especially for burnt people and giant nevi. According to our knowledge in modelling pigmented skin, we decided develop an artificial, autologous skin therapeutic use. Our models were made on dead deepidermized dermis, which do not allow standardization procedure. Thus, since dermal collagen-based matrices such as IntegraTM or Matriderm® used substitute from many years reconstructive surgery, artificial these matrices. Fibroblasts, keratinocytes melanocytes isolated breast obtained after written informed consent donors culture clinical grade medium. Fibroblasts seeded top the bovine matrix 7 days prior seeding. After 3 weeks, a with remodeled epidermal junction stratified epidermis its stratum corneum, named Geniouskin. The process was repeatable reproducible patented (EP3072535). A preclinical study performed confirm suitability Quality Geniouskin altered 6 hours transport at temperature between 20-25°C. Four series mice grafted prepared 4 various phototypes. Grafts behavior followed during months. increase body weight male female indicates no obvious toxicity No specific sign correlated graft. well integrated induced deleterious side effects (tumor, inflammation).By immunohistochemistry HE staining observed that integrated, differentiated, pigmented, vascularized innervated confirmed ability replace efficiently native skin.